Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Production of High-protein Hydrolysate from Poultry Industry Residue and their Molecular Profiles

2009; Taylor & Francis; Volume: 23; Issue: 3 Linguagem: Inglês

10.1080/08905430903102828

1532-4249

Daniele Misturini Rossi, Simone Hickmann Flôres, Júlio Xandro Heck, Marco Antônio Záchia Ayub,

Proteins in Food Systems

The use of mechanically deboned poultry meat (MDPM) as a source of raw protein for the enzymatic preparation of hydrolysates was investigated using commercial proteases Alcalase and Flavourzyme. Hydrolysis conditions were optimized by response surface methodology, and the influence of temperature, pH, and enzyme concentration were evaluated to determine the hydrolysis index. For Alcalase, optimal values of temperature, pH, and enzyme concentration were found to be 50°C, 7.5%, and 2.5%, respectively, while for Flavourzyme the optimized variables were at 50°C, 6.0%, and 3.5%, respectively. Compared to raw MDPM, Alcalase produced a very high recovery of 89% of the nitrogen from the hydrolysates, while for Flavourzyme recovery was around 67%. Alcalase was used to produce a spray-dried hydrolysate containing 62% of total protein with molecular weights ranging from 5,807 to 12,000 Da, and had excellent microbiological quality.