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Genetic Relationships among Korean Brown Frog Species (Anura, Ranidae), with Special Reference to Evolutionary Divergences between Two Allied Species Rana dybowskii and R. huanrenensis

2002; Zoological Society of Japan; Volume: 19; Issue: 3 Linguagem: Inglês

10.2108/zsj.19.369

2212-3830

Jong‐Bum Kim, Mi‐Sook Min, Suh‐Yung Yang, Masafumi Matsui,

Molecular Biology Techniques and Applications

Allozyme analysis for 41 populations of brown frog species, Rana dybowskii, R. huanrenensis, and R. amurensis from Korea and three reference species (Chinese R. chensinensis and Japanese R. dybowskii and R. tsushimensis), were performed to clarify taxonomic status of Korean brown frogs. The level of average genetic differentiation (Nei's D) among local populations of each species in Korea was very low (D 0.370). In the genetic relationships among five species examined, the 24 chromosome brown frogs (R. dybowskii, R. huanrenensis, and R. chensinensis) did not form a monophyletic group. Rana dybowskii with the chromosome number of 2n=24 was grouped together with R. amurensis with the chromosome number of 2n=26. The hypothesis of reversal change from 24 to 26 in Korean R. amurensis seems to better explain the phylogenetic relationships of east Asian brown frogs than the assumption of parallel reduction in chromosome number from 2n=26 to 24 in R. dybowskii and in the common ancestor of R. huanrenensis and R. chensinensis. The genetic, morphological, and reproductive divergences between Korean R. dybowskii and R. huanrenensis were compared.