Detection and Serotyping of Streptococcus pneumoniae from Nasopharyngeal Samples by PCR-Based Multiplex Assay
2005; American Society for Microbiology; Volume: 43; Issue: 12 Linguagem: Inglês
10.1128/jcm.43.12.6152-6154.2005
ISSN1098-660X
AutoresJaime Moreno, Elkin Hernández, Olga Sanabria, Elizabeth Castañeda,
Tópico(s)Bacterial Infections and Vaccines
ResumoABSTRACT We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.
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