Multidimensional CP-MAS 13C NMR of uniformly enriched chlorophyll
1998; Elsevier BV; Volume: 54; Issue: 9 Linguagem: Inglês
10.1016/s1386-1425(98)00067-5
ISSN1873-3557
AutoresBarth‐Jan van Rossum, G.J. Boender, Fokko M. Mulder, Jan Raap, Teodor Silviu Balaban, Alfred R. Holzwarth, Kurt Schaffner, S. Prytulla, Hartmut Oschkinat, Huub J. M. de Groot,
Tópico(s)Solid-state spectroscopy and crystallography
ResumoThe progress toward structure refinement of solid-type uniformly 13C enriched ([U-13C]) chlorophyll-containing biological preparations is summarised. Solid state carbon chemical shifts of aggregated [U-13C] bacteriochlorophyll (BChl) c in intact chlorosomes of Chlorobium tepidum and in [U-13C] BChl c aggregates were determined by the application of homonuclear (13C13C) magic angle spinning (MAS) NMR dipolar correlation spectroscopy. It was found that the arrangement of BChl c molecules in the chlorosomes and in the aggregates is highly similar, which provides convincing evidence that self-organisation of the BChl c is the main mechanism to support the structure of the chlorosomes. Additionally, high field 2-D (1H13C) and 3-D (1H13C13C) dipolar correlation spectroscopy was applied to determine solid state proton chemical shifts of aggregated [U-13C] BChl c in intact chlorosomes. From the high-field assignments, evidence is found for the existence of at least two well-defined interstack arrangements.
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