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Graph Based Study of Allergen Cross-Reactivity of Plant Lipid Transfer Proteins (LTPs) Using Microarray in a Multicenter Study

2012; Public Library of Science; Volume: 7; Issue: 12 Linguagem: Inglês

10.1371/journal.pone.0050799

1932-6203

Arantxa Palacín, Cristina Gómez‐Casado, Luis A. Rivas, Jacobo Aguirre, Leticia Tordesillas, Joan Bartra, Carlos Blanco, Teresa Carrillo, Javier Cuesta‐Herranz, Consolación De Frutos, Genoveva García Álvarez-Eire, Javier Fernández, P M Gamboa, Rosa Muñoz‐Cano, Rosa Sánchez‐Monge, Sofía Sirvent, Marı́a José Torres, Susana Varela-Losada, Rosalı́a Rodrı́guez, Vı́ctor Parro, Miguel Blanca, Gabriel Salcedo, Araceli Díaz‐Perales,

Contact Dermatitis and Allergies

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.