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Development of 15 polymorphic microsatellite markers in the Atlantic tarpon ( Megalops atlanticus ) for capture–recapture studies

2007; Wiley; Volume: 8; Issue: 1 Linguagem: Inglês

10.1111/j.1471-8286.2007.01924.x

1755-0998

Seifu Seyoum, Michael D. Tringali, Maryanne Higham,

Identification and Quantification in Food

Fifteen polymorphic microsatellite DNA loci for Atlantic tarpon, Megalops atlanticus, were isolated by using PIMA, a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to 24 (mean = 7.7) in 65 specimens from Tampa Bay, Florida. Observed and expected heterozygosities ranged from 0.27 to 0.92 (mean = 0.60) and from 0.28 to 0.95 (mean = 0.62), respectively. Genotypes at one locus deviated significantly from Hardy-Weinberg equilibrium. In exact tests for genotypic disequilibrium, there was no evidence of associations between any pair of loci. Overall, loci were well resolved and highly polymorphic, confirming their suitability for DNA fingerprinting applications and other genetic studies.