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Transgenic modification of the n-3 HUFA biosynthetic pathway in nibe croaker larvae: improved DPA (docosapentaenoic acid; 22:5n-3) production

2015; Wiley; Volume: 22; Issue: 2 Linguagem: Inglês

10.1111/anu.12273

1365-2095

Naoki Kabeya, Yutaka Takeuchi, Ryosuke Yazawa, Yutaka Haga, Shuichi Satoh, Goro Yoshizaki,

Animal Genetics and Reproduction

Aquaculture NutritionVolume 22, Issue 2 p. 472-478 Original Article Transgenic modification of the n-3 HUFA biosynthetic pathway in nibe croaker larvae: improved DPA (docosapentaenoic acid; 22:5n-3) production N. Kabeya, N. Kabeya Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, Japan Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorY. Takeuchi, Y. Takeuchi Research Center for Advanced Science and Technology, Tokyo University of Marine Science and Technology, Tateyama-shi, Chiba, JapanSearch for more papers by this authorR. Yazawa, R. Yazawa Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorY. Haga, Y. Haga Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorS. Satoh, S. Satoh Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorG. Yoshizaki, Corresponding Author G. Yoshizaki Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanCorrespondence: G. Yoshizaki, Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. E-mail: goro@kaiyodai.ac.jpSearch for more papers by this author N. Kabeya, N. Kabeya Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, Japan Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorY. Takeuchi, Y. Takeuchi Research Center for Advanced Science and Technology, Tokyo University of Marine Science and Technology, Tateyama-shi, Chiba, JapanSearch for more papers by this authorR. Yazawa, R. Yazawa Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorY. Haga, Y. Haga Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorS. Satoh, S. Satoh Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanSearch for more papers by this authorG. Yoshizaki, Corresponding Author G. Yoshizaki Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, JapanCorrespondence: G. Yoshizaki, Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. E-mail: goro@kaiyodai.ac.jpSearch for more papers by this author First published: 06 January 2015 https://doi.org/10.1111/anu.12273Citations: 7Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Marine fish are generally unable to produce sufficient quantities of n-3 highly unsaturated fatty acid (n-3 HUFA) such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). Consequently, the seed production of marine fish requires careful nutritional enrichment of live feeds such as rotifers and brine shrimp Artemia to meet n-3 HUFA requirements for normal growth. Another strategy for improving n-3 HUFA availability is modifying the biosynthetic pathway of marine fish using transgenic technology. In this study, we conducted a feeding trial with non-transgenic and transgenic nibe croaker Nibea mitsukurii carrying the elongation of very long-chain fatty acids protein 2 (Elovl2) gene isolated from masu salmon Oncorhynchus masou and three groups of Artemia (non-enriched and enriched with two products). For all Artemia groups, docosapentaenoic acid (DPA, 22:5n-3), which is a direct product of Elovl2, was significantly higher in the transgenic fish than that in non-transgenic fish, despite the absence of DPA in all diets. Thus, applying transgenic techniques to marine fish at the larval stage are a powerful strategy for modifying n-3 HUFA biosynthetic pathways. Citing Literature Volume22, Issue2April 2016Pages 472-478 RelatedInformation