LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides
2007; Royal Society of Chemistry; Volume: 5; Issue: 15 Linguagem: Inglês
10.1039/b706101c
ISSN1477-0539
AutoresTorben Højland, Surender Kumar, B. Ravindra Babu, T. Umemoto, Nanna Albæk, Pawan K. Sharma, Poul Nielsen, Jesper Wengel,
Tópico(s)RNA Interference and Gene Delivery
ResumoThe triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2′-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2′-functionalisation of 2′-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2′,C3′-oxymethylene linker (E-type furanose conformation) or a C2′,C4′-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.
Referência(s)