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Isolation and molecular identification of Leishmania ( Viannia ) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes

2007; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 102; Issue: 5 Linguagem: Inglês

10.1590/s0074-02762007005000077

1678-8060

Jeanneth Pérez, Nicolás Veland, Diego A. Espinosa, Katherine Torres, Elena Ogusuku, Alejandro Llanos‐Cuentas, Dionicia Gamboa, Jorge Arévalo,

Insect Resistance and Genetics

Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.