Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Determinação de lamivudina, estavudina e nevirapina, em comprimidos, por cromatografia líquida de alta eficiência

2006; Brazilian Chemical Society; Volume: 29; Issue: 6 Linguagem: Inglês

10.1590/s0100-40422006000600002

1678-7064

Gisele Rodrigues da Silva, Gustavo Portela Lages, Gérson Antônio Pianetti, Elzíria de Aguiar Nunan, Cristina Duarte Vianna‐Soares, Lígia Maria Moreira de Campos,

HIV/AIDS drug development and treatment

PERFORMANCE LIQUID CHROMATOGRAPHY.A high performance liquid chromatography method was developed to quantify lamivudine, stavudine and nevirapine combined in tablets.The separation was carried out in less than 10 min using a phosphate buffer of pH 3.0 and acetonitrile (75:25, v/v) as mobile phase, a LiChrospher ODS column and UV detection at 266 nm.The method was linear over the range of 15-135 μg/mL (lamivudine), 4-36 μg/mL (stavudine) and 20-180 μg/mL (nevirapine).The accuracy ranged from 98.56 to 102.04% and intra-day and inter-day precision was less than 1% for the three drugs.The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.