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Recombinant Influenza A Viruses with Enhanced Levels of PB1 and PA Viral Protein Expression

2012; American Society for Microbiology; Volume: 86; Issue: 10 Linguagem: Inglês

10.1128/jvi.06384-11

1098-5514

Alan Belicha‐Villanueva, Juan R. Rodríguez-Madoz, Jad Maamary, Alina Baum, Dabeiba Bernal‐Rubio, Maria Minguito de la Escalera, Ana Fernandez‐Sesma, Adolfo Garcı́a-Sastre,

Respiratory viral infections research

ABSTRACT Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.