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Multiplexed Antibody Detection With an Array of Silicon-on-Insulator Microring Resonators

2009; Institute of Electrical and Electronics Engineers; Volume: 1; Issue: 4 Linguagem: Inglês

10.1109/jphot.2009.2035433

1943-0655

Katrien De Vos, Jordi Gironès, Tom Claes, Yannick De Koninck, Stanislav Popelka, E. Schacht, Roel Baets, Peter Bienstman,

Advanced Biosensing Techniques and Applications

Optical cavities are considered promising devices for biosensing to satisfy the high demands of the growing proteomics market.We present a prototype sensor for multiplexed label-free monitoring of biomolecular interactions based on an array of three-by-four silicon-oninsulator microring resonators fabricated by standard complementary metal-oxide semicondutor (CMOS) technology.Parallel readout was performed with an infrared camera.We have demonstrated the simultaneous detection of several model antibodies in a highly specific way.For that, we integrated the optical chip with low-temperature polydimethylsiloxane (PDMS) packaging, enabling excellent compatibility with the receptor molecules.We obtain a very selective biomolecular interaction by grafting a 2.5-nm poly(ethylene glycol) layer to the silicon.Using silicon on insulator as the material platform offers high sensitivity through miniaturization and low-cost mass fabrication through standard CMOS technology steps.The sensors in this interrogation method are able to detect 3.4 pg=mm 2 of a protein overlayer or a theoretical total mass of about 74 ag.For this novel lab on a chip, the fabrication, chemistry, readout, and packaging are developed to be scalable to detect hundreds of affinity interactions within several minutes.