Portal de Periódicos da CAPES

Cystinosin, the protein defective in cystinosis, is a H+-driven lysosomal cystine transporter

2001; Springer Nature; Volume: 20; Issue: 21 Linguagem: Inglês

10.1093/emboj/20.21.5940

1460-2075

Vasiliki Kalatzis,

Neonatal Health and Biochemistry

Article1 November 2001free access Cystinosin, the protein defective in cystinosis, is a H+-driven lysosomal cystine transporter Vasiliki Kalatzis Vasiliki Kalatzis INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Stéphanie Cherqui Stéphanie Cherqui INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Corinne Antignac Corresponding Author Corinne Antignac INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Bruno Gasnier Corresponding Author Bruno Gasnier CNRS UPR 1929, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France Search for more papers by this author Vasiliki Kalatzis Vasiliki Kalatzis INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Stéphanie Cherqui Stéphanie Cherqui INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Corinne Antignac Corresponding Author Corinne Antignac INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France Search for more papers by this author Bruno Gasnier Corresponding Author Bruno Gasnier CNRS UPR 1929, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France Search for more papers by this author Author Information Vasiliki Kalatzis1, Stéphanie Cherqui1, Corinne Antignac 1 and Bruno Gasnier 2 1INSERM U423, Université René Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France 2CNRS UPR 1929, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France *Corresponding authors. E-mail: [email protected] or E-mail: [email protected] The EMBO Journal (2001)20:5940-5949https://doi.org/10.1093/emboj/20.21.5940 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cystinosis is an inherited lysosomal storage disease characterized by defective transport of cystine out of lysosomes. However, the causative gene, CTNS, encodes a seven transmembrane domain lysosomal protein, cystinosin, unrelated to known transporters. To investigate the molecular function of cystinosin, the protein was redirected from lysosomes to the plasma membrane by deletion of its C-terminal GYDQL sorting motif (cystinosin-ΔGYDQL), thereby exposing the intralysosomal side of cystinosin to the extracellular medium. COS cells expressing cystinosin-ΔGYDQL selectively take up L-cystine from the extracellular medium at acidic pH. Disruption of the transmembrane pH gradient or incubation of the cells at neutral pH strongly inhibits the uptake. Cystinosin-ΔGYDQL is directly involved in the observed cystine transport, since this activity is highly reduced when the GYDQL motif is restored and is abolished upon introduction of a point mutation inducing early-onset cystinosis. We conclude that cystinosin represents a novel H+-driven transporter that is responsible for cystine export from lysosomes, and propose that cystinosin homologues, such as mammalian SL15/Lec35 and Saccharomyces cerevisiae ERS1, may perform similar transport processes at other cellular membranes. Introduction Cystinosis is a lysosomal storage disease characterized by an intralysosomal accumulation of cystine, which is due to defective cystine efflux from these organelles. This autosomal recessive disorder comprises three allelic clinical forms, varying in severity and age of onset. The infantile form (MIM 21980) generally appears at 6–8 months of age with a proximal renal tubulopathy, which, in the absence of renal transplantation, can lead to death by 10 years of age due to renal failure (for review see Gahl et al., 1995). Other clinical signs, notably retinal blindness, hypothyroidism, diabetes mellitus, swallowing difficulties and neurological deterioration, eventually appear due to the widespread accumulation of cystine in most tissues. The juvenile form (MIM 219900) is characterized by glomerular renal damage, which manifests at around 10–12 years of age and slowly progresses to glomerular insufficiency and photophobia due to corneal cystine-crystal deposits. Finally, the ocular non-nephropathic form (MIM 219750) is solely characterized by a mild photophobia but no renal anomalies. Cystine, the disulfide of the amino acid cysteine, is a by-product of lysosomal protein hydrolysis, and is reduced to cysteine in the cytoplasm. As the enzymes involved in cyst(e)ine redox reactions are normal in cystinotic cells, it has been hypothesized that the underlying metabolic defect of cystinosis is a defective lysosomal membrane cystine transport (Gahl et al., 1995). Support for this hypothesis has been provided by the demonstration that cystine is rapidly lost from artificially loaded normal lysosomes, whereas cystine efflux from cystinotic lysosomes is almost non-existent (Gahl et al., 1982b; Jonas et al., 1982a, b; Steinherz et al., 1982). Furthermore, it has been shown that lysosomal cystine transport is carrier mediated due to the observation that the velocity of cystine egress from normal lysosomes shows saturation kinetics (Gahl et al., 1982a) and the demonstration of cystine countertransport across the normal lysosomal membrane (Gahl et al., 1983)—two hallmarks of carrier-mediated transport. The gene underlying cystinosis, CTNS, was identified using a positional cloning strategy (Town et al., 1998). CTNS encodes a 367 amino acid protein, cystinosin, which comprises seven predicted transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a 10 amino acid cytosolic C-terminus containing a tyrosine-based lysosomal sorting motif (GYDQL). We recently demonstrated that cystinosin is indeed a lysosomal membrane protein and that its targeting requires the presence of the GYDQL motif, in particular the Y and L residues, as well as a novel sorting motif localized in the third cytoplasmic loop (Cherqui et al., 2001). Deletion of one or the other of these targeting signals partially redirects cystinosin to the plasma membrane in addition to lysosomes, whereas deletion of both sorting motifs completely redirects cystinosin to the plasma membrane. Although all forms of cystinosis have been linked to mutations in cystinosin (Shotelersuk et al., 1998; Town et al., 1998; Attard et al., 1999; Thoene et al., 1999), it has not yet been determined whether this protein is directly or indirectly responsible for the defective cystine transport of cystinotic lysosomes. Cystinosin may represent the lysosomal cystine transporter itself. However, neither its sequence nor its predicted topology displays a similarity to currently known transporters. It has thus been alternatively proposed that cystinosin might indirectly influence lysosomal cystine efflux (Attard et al., 1999; Mancini et al., 2000). In this study, we addressed this issue by using a strategy that exploited our recent knowledge of the signals involved in the targeting of cystinosin to lysosomes. As the lysosomal lumen is not easily accessible for transport experiments (Pisoni and Schneider, 1992), the recombinant protein was redirected to the plasma membrane by mutation of its C-terminal tyrosine-based motif (Cherqui et al., 2001), thereby creating a cellular model in which the ability of cystinosin to translocate cystine could be examined using whole cells. This experimental approach allowed us to characterize the molecular function of cystinosin. Results Cystinosin is a cystine transporter We recently characterized several artificial mutations of cystinosin that redirect the recombinant protein to the plasma membrane in different cell lines (Cherqui et al., 2001). To test the potential transport activity of cystinosin, a mutant (ΔGYDQL) in which the C-terminal tyrosine-based motif is deleted was expressed in COS cells, and the ability of transfected cells to take up [35S]L-cystine from the extracellular medium was examined. Such an accumulation is equivalent to an efflux of cystine from lysosomes because the extracellular medium is topologically equivalent to the lysosomal lumen: in both processes, cystine is translocated towards the cytosol (Figure 1). Figure 1.Rationale used for the cystine transport assay of cystinosin. In vivo, wild-type cystinosin is localized at the lysosomal membrane (smaller grey circle) and is thought to transport cystine (C-S-S-C) from the lysosomal lumen to the cytosol. In our experimental model, we have deleted the C-terminal lysosomal targeting signal from cystinosin (cystinosin-ΔGYDQL). This results in a partial redirection of this protein to the plasma membrane (larger grey circle) in transfected COS cells. In this model, the extracellular medium is topologically equivalent to the lysosomal lumen, and cystinosin-ΔGYDQL would thus act to transport [35S]cystine from the extracellular medium into the cytosol. Download figure Download PowerPoint In a standard extracellular medium (buffer A, pH 7.4; Materials and methods), a modest increase in accumulated [35S]cystine was often observed in cells expressing the ΔGYDQL mutant compared with those mock transfected with a plasmid without an insert ('background' level) or expressing wild-type cystinosin (Figure 2A). A mean increase of 39 ± 11% (± SEM) over background was obtained from 18 independent experiments. In order to mimic the acidic lumen of lysosomes, the extracellular pH was decreased to 5.6 during the incubation with [35S]cystine (buffer B). This acidification dramatically increased the amount of [35S]cystine accumulated in cystinosin-ΔGYDQL-expressing cells, but not in mock-transfected cells (Figure 2A). A mean value of 635 ± 50% over background was obtained from 45 independent experiments with the ΔGYDQL mutant. This acidification also revealed a low [35S]cystine uptake activity associated with the expression of wild-type cystinosin (131 ± 33% over background; n = 9), in agreement with a faint localization of the wild-type protein at the plasma membrane in addition to lysosomes (see below and Figure 8C). Figure 2.Cystine uptake ability of cystinosin-ΔGYDQL-expressing cells. (A) Assay of transfected cells for [35S]cystine uptake in a neutral (pH 7.4, hatched bars) or acidic (pH 5.6, grey bars) extracellular medium. At neutral pH, cells expressing cystinosin-ΔGYDQL show a modest increase in the amount of accumulated [35S]cystine as compared with mock-transfected cells or wild-type cystinosin-expressing cells. At acidic pH, a dramatic increase in accumulated [35S]cystine is observed in cystinosin-ΔGYDQL-expressing cells but not in mock-transfected cells. A small amount of [35S]cystine is also taken up by wild-type cystinosin-expressing cells. Error bars correspond to the SEM for all figures. (B) Cystinosin-ΔGYDQL-mediated [35S]cystine uptake (black squares) remained linear for 10 min. [35S]cystine uptake mediated by mock-transfected cells is indicated by white squares. (C) Amount of accumulated [35S]cystine remaining after a 3 and a 6 min incubation with 20 μM digitonin treatment of mock-transfected (white squares) or cystinosin-ΔGYDQL-expressing (black squares) cells. Download figure Download PowerPoint The cystinosin-ΔGYDQL-mediated [35S]cystine uptake remained linear for 10 min (Figure 2B). We thus used a duration of ≤10 min throughout this study to measure uptake velocities. To determine whether the cystinosin- ΔGYDQL-induced cystine uptake reflected translocation across the plasma membrane or binding to the cell surface, cells exposed to [35S]cystine were subsequently incubated with 20 μM digitonin, a detergent that selectively permeabilizes the plasma membrane (Zuurendonk and Tager, 1974; Fiskum et al., 1980). Digitonin treatment rapidly released the accumulated radioactivity (Figure 2C), which is in agreement with a transport mechanism. Taken together, these data demonstrate that the expression of cystinosin at the plasma membrane is associated with the induction of a cystine transport activity across this membrane. The simplest interpretation is that cystinosin does transport cystine (see Discussion). Cystine transport is driven by a proton transmembrane gradient Cystinosin-ΔGYDQL-mediated cystine transport was stimulated at acidic extracellular pH (Figure 2A). To characterize the pH effect further, cells were incubated with [35S]cystine in buffers ranging from pH 7.4 to 5.6. Cystine uptake by cystinosin-ΔGYDQL increased with decreasing pH, but without reaching a plateau (Figure 3A). This increase may depend exclusively on the pH of the extracellular compartment or, since cells maintain their cytosol at neutral pH, it may have resulted from the artificial transmembrane pH gradient created by the change in extracellular buffer. To discriminate between these hypotheses, the ionophore nigericin was added to the 'acidic' uptake buffer (buffer B, pH 5.6). Nigericin exchanges K+ for H+ (Pressman, 1968) and is widely used to dissipate transmembrane pH gradients. As shown in Figure 3B, 5 μM nigericin inhibited [35S]cystine uptake by cystinosin-ΔGYDQL by >85% at pH 5.6, demonstrating that the cystine transport is driven by the pH gradient. This observation suggests that cystinosin operates as a H+ symporter, i.e. that it couples the translocation of cystine to a translocation of H+ in the same direction. With the exception of Figure 7, all the experiments reported hereafter were performed at pH 5.6. Figure 3.Effect of a transmembrane pH gradient on cystine uptake. (A) [35S]cystine uptake by mock-transfected (white squares) and cystinosin-ΔGYDQL (black squares) expressing cells was performed in standard uptake buffer (see Materials and methods) adjusted to a pH ranging from 5.6 to 7.4 with 20 mM potassium phosphate. Cystinosin-mediated uptake increased with decreasing pH. (B) Amount of [35S]cystine accumulated by mock-transfected, cystinosin-expressing and cystinosin-ΔGYDQL-expressing cells in an acidic extracellular medium (grey boxes). The addition of 5 μM nigericin to the uptake media (striped boxes) abolished [35S]cystine uptake by cystinosin and cystinosin-ΔGYDQL, demonstrating the dependence of cystine transport on a proton gradient. (C) Effect of the presence of extracellular Na+ and K+ (uptake media containing NaCl as the major osmolyte, buffered with potassium phosphate K+-Pi), solely K+ (sucrose, Pi-K+) and solely Na+ (sucrose, MES-Na+) on [35S]cystine uptake by mock-transfected (white bars) and cystinosin-ΔGYDQL-expressing (black bars) cells. These changes do not significantly alter the amount of [35S]cystine taken up by cystinosin-ΔGYDQL, demonstrating that cystine transport does not require other ions. Download figure Download PowerPoint Figure 4.Saturation kinetics of cystinosin-mediated cystine uptake. (A) Plot of the velocity (V) of [35S]cystine uptake versus increasing substrate concentration (S) for mock-transfected (white squares) and cystinosin-ΔGYDQL-expressing (black squares) cells over an 8 min uptake period (linear phase). A deduction of background levels demonstrates that cystine uptake by cystinosin-ΔGYDQL is saturable (black triangles). (B) A linear Eadie–Hostee plot of the cystinosin-ΔGYDQL-dependent data demonstrates that cystine transport follows Michaelis–Menten kinetics. KM = 350 μM and Vmax = 507 pmol/min per well for this experiment. Download figure Download PowerPoint Figure 5.Stereoselectivity of cystine uptake. Assay of [35S]cystine uptake by mock-transfected (white bars) or cystinosin-ΔGYDQL (black bars) expressing cells in the absence (control) or presence of 600 μM L- or D-cystine. L-cystine inhibits [35S]cystine uptake by cystinosin-ΔGYDQL by >60%, whereas its stereoisomer D-cystine has no significant effect. Download figure Download PowerPoint Figure 6.Cysteine uptake ability of cystinosin-ΔGYDQL-expressing cells. (A) [35S]cystine accumulated by cystinosin-ΔGYDQL in the presence of increasing concentrations of L-cysteine (logarithmic scale) is expressed as a percentage of uptake in the absence of cysteine. Half-inhibition of [35S]cystine uptake was obtained for a cysteine concentration of 1.5 mM, a value ∼5-fold higher than the cystine concentration that half-saturates cystinosin (278 ± 49 μM). (B) At equal concentrations, L-cystine inhibits [35S]cystine uptake by cystinosin-ΔGYDQL (black bars), whereas L-cysteine has no effect. [35S]cystine uptake by mock-transfected cells is shown as white bars. (C) At equal substrate occupancy (i.e. in the presence of a 5-fold higher concentration of [35S]cysteine as opposed to [35S]cystine), cystinosin does not translocate cysteine significantly. Bars as for (B). Download figure Download PowerPoint Figure 7.Effect of the cystinotic point mutation G308R on cystine uptake. Amount of [35S]cystine accumulated by mock-transfected, cystinosin-ΔGYDQL-expressing and cystinosin-G308R-ΔGYDQL-expressing cells in a neutral (hatched bars) and acidic (grey bars) uptake medium. The introduction of the G308R point mutation associated with infantile cystinosis abolishes cystine transport by cystinosin-ΔGYDQL. Download figure Download PowerPoint Figure 8.Effect of G308R on the amount of recombinant protein produced or its subcellular localization. (A) Amount of [35S]cystine accumulated by cells expressing GFP or the fusion proteins cystinosin–GFP, cystinosin-ΔGYDQL–GFP and cystinosin-G308R-ΔGYDQL–GFP in a neutral (hatched bars) and acidic (grey bars) uptake medium. (B) Western blot analysis of the same lot of transfected cells using an anti-GFP monoclonal antibody demonstrates that cystinosin-G308R-ΔGYDQL–GFP is not produced at a lower level than cystinosin–GFP or cystinosin-ΔGYDQL–GFP. (C) Immunofluorescence studies on the same lot of transfected cells demonstrate that cystinosin-ΔGYDQL–GFP and cystinosin-G308R-ΔGYDQL–GFP have the same subcellular localization pattern, and that both of these fusion proteins are present at a much higher level at the plasma membrane than cystinosin–GFP. Scale bar 40 μm for all panels. Download figure Download PowerPoint To examine whether other extracellular ions influence the cystinosin-ΔGYDQL-mediated cystine transport, NaCl in buffer B was replaced by an isotonic concentration of KCl (data not shown) or sucrose (Figure 3C) and the potassium phosphate buffer was replaced by an equivalent amount of 2-(N-morpholino) ethane sulfonate (MES) adjusted to the same pH (Figure 3C). These changes did not significantly alter [35S]cystine uptake by cystinosin- ΔGYDQL; therefore, the transmembrane proton gradient appears to be the sole driving force of cystine transport. Substrate selectivity of the transport activity In order to characterize the interaction of cystinosin with cystine, cystinosin-ΔGYDQL-expressing cells were incubated with increasing concentrations of [35S]cystine for 8 min. Cells took up [35S]cystine at a constant rate over this time period at all concentrations tested (data not shown). The uptake velocity increased linearly up to 100 μM (Figure 4A). Above 100 μM, the increase in velocity declined progressively, suggesting a saturation of kinetics. A linear Eadie–Hofstee plot of the cystinosin-ΔGYDQL-dependent data confirmed that the cystine transport is saturable and follows Michaelis–Menten kinetics (Figure 4B). A KM of 278 ± 49 μM was calculated from three independent transfections. The Vmax, which depends on the transfection efficiency, ranged between 108 and 507 pmol/min per well (mean value: 316 ± 116 pmol/min per well). To address the specificity of cystinosin for cystine, we compared the ability of this transporter to take up 40 μM [35S]L-cystine in the presence of 600 μM cold L- or D-cystine (limiting experimental concentration due to cystine insolubility). L-cystine inhibited [35S]L-cystine uptake by >60%, whereas D-cystine did not inhibit cystine uptake significantly (Figure 5). The intralysosomal hydrolysis of proteins generates a variety of amino acids, which may interfere with the cystinosin-mediated cystine efflux. The ability of diverse classes of amino acids to inhibit [35S]L-cystine uptake was therefore tested using a 10 mM concentration of each amino acid (Table I). This concentration greatly exceeds the concentration found in the lysosomal lumen, which occurs in the 10–100 μM range for each amino acid (Vadgama et al., 1991). Therefore, only major effects are expected to be relevant in vivo. L-methionine, L-leucine, L-alanine and L-valine did not inhibit cystinosin-ΔGYDQL-mediated cystine uptake by >40% (Table I). L-serine and L-threonine also inhibited cystine uptake only moderately. Phenylalanine did not significantly inhibit cystine uptake, and L-proline and L-glutamic acid did not inhibit uptake at all. In contrast, L-cysteine, when present at a 10 mM concentration, inhibited cystine uptake by 75%. Therefore, among all amino acids tested, only cysteine appeared as a possible additional substrate. Table 1. Effect of amino acids on [35S]L-cystine accumulation Amino acid (10 mM) % uninhibited± SEM Greene et al. (1990) L-methionine 73 ± 12 (n = 4) 51 ± 4 L-leucine 75 ± 12 (n = 4) 61 ± 4 L-alanine 69 ± 9 (n = 6) 86 L-valine 60 ± 7 (n = 6) 66 L-phenylalanine 87 ± 7 (n = 6) 78 L-proline 102 ± 9 (n = 6) 87 L-serine 81 ± 4 (n = 4) 79 L-threonine 69 ± 7 (n = 6) 104 L-cysteine 25 ± 4 (n = 7) n.d. L-glutamic acid 102 ± 11 (n = 4) n.d. Values are expressed as the mean ± SEM of n independent observations, and are compared with those obtained by Greene et al. (1990) for the lysosomal cystine countertransport activity of mouse L-929 fibroblasts. n.d., not determined. The effect of increasing concentrations of L-cysteine on cystinosin-ΔGYDQL-mediated cystine uptake was then tested. Half-inhibition was obtained for 1.5 mM cysteine (Figure 6A), a value 5-fold higher than the cystine concentration that half-saturates cystinosin (Figure 4). The fact that 600 μM L-cystine inhibited ∼65% of the [35S]cystine transport, whereas an identical concentration of L-cysteine had no effect, confirmed that cystinosin preferentially recognizes L-cystine (Figure 6B). To examine whether cysteine molecules bound to cystinosin are translocated across the cell membrane, cystinosin-ΔGYDQL-expressing cells were incubated with 200 μM [35S]L-cysteine at pH 5.6. For comparison, a parallel set of cells was incubated with a 5-fold lower concentration of [35S]cystine to compensate for the 5-fold higher affinity of cystine relative to cysteine. In such conditions, if [35S]cysteine and [35S]cystine were translocated by cystinosin with equal molecular turnovers, identical uptake signals (in picomoles) should be observed. This proved not to be the case, as although 201 ± 37 pmol/well [35S]cystine were taken up by cystinosin-ΔGYDQL, identical levels of 383 ± 58 and 378 ± 42 pmol/well [35S]cysteine were detected in cells expressing and not expressing cystinosin-ΔGYDQL, respectively (Figure 6C). In this experiment, background levels of cysteine uptake were decreased by substituting K+ for Na+ in buffer B (Figure 6C) in order to detect a possible cystinosin-mediated translocation of cysteine with more sensitivity. In further experiments, this background level was further reduced ∼2-fold by the addition of 5 mM serine or alanine, in agreement with the existence of an endogenous alanine/serine/cysteine transporter (ASCT) in COS cells (Palacin et al., 1998). However, even under these conditions, which did not significantly inhibit the cystinosin-mediated [35S]cystine uptake (Figure 3C; Table I), no cystinosin-dependent uptake of [35S]cysteine could be detected (data not shown). These experiments show that, at equal substrate occupancy, cystinosin translocates cysteine less efficiently, if at all, than cystine. Taken together, these data show that cystinosin is highly selective for cystine. A point mutation causing severe cystinosis abolishes cystine transport activity Most of the mutations detected in patients with early-onset cystinosis are loss of function mutations, such as a large 57 kb (Touchman et al., 2000) deletion spanning almost the entire length of the gene (Town et al., 1998) and underlying 75% of European cases of infantile cystinosis (Forestier et al., 1999). Missense mutations have also been identified, and these are generally clustered in the C-terminal region of cystinosin (Attard et al., 1999). We tested the effect of one such mutation, G308R, detected in several affected individuals (Shotelersuk et al., 1998; Attard et al., 1999) and which substitutes an arginine for a highly conserved glycine residue in the sixth transmembrane domain, on cystine transport. G308R was introduced into the construct carrying the ΔGYDQL mutant and we found that cystine transport was reduced to background levels (Figure 7). In order to verify that this effect was not due to an alteration in the amount of cystinosin that reaches the plasma membrane, we used western blot analysis and immunofluorescence studies of cystinosin constructs fused to a C-terminal green fluorescent protein (GFP) tag (Cherqui et al., 2001). Transport studies, western blot analysis and immunofluorescent studies were carried out on the same lot of transfected cells. Expression of the cystinosin-ΔGYDQL–GFP fusion protein in COS cells induced a strong cystine uptake activity at pH 5.6 (Figure 8A), showing that the fusion to GFP did not preclude the transport activity of cystinosin. A more limited activity was detected with the wild-type cystinosin–GFP fusion protein, as observed for the untagged protein (Figure 2A). Although the overall amount of wild-type cystinosin–GFP expressed was higher than that of cystinosin-ΔGYDQL–GFP (Figure 8B), the reduced activity of the wild-type protein could be correlated with a reduced expression at the plasma membrane of COS cells (Figure 8C). In contrast, the introduction of the G308R mutation into the cystinosin-ΔGYDQL–GFP protein abolished the cystine transport activity (Figure 8A), but did not reduce its overall level of expression (Figure 8B), nor did it significantly alter its localization at the plasma membrane (Figure 8C). These data show that the cystinotic point mutation prevents cystinosin from translocating cystine. Discussion Cystinosin is the lysosomal cystine transporter The lysosomal storage disorder cystinosis is characterized by an intralysosomal accumulation of cystine due to a defective cystine efflux from lysosomes (Gahl et al., 1995). The causative gene was identified (Town et al., 1998) and the encoded protein, cystinosin, was recently shown to be a lysosomal membrane protein (Cherqui et al., 2001). However, the molecular function of cystinosin has remained unknown. By deleting the GYDQL lysosomal sorting motif (Cherqui et al., 2001), we were able to partially redirect cystinosin to the plasma membrane, generating a cellular model in which the lysosomal side of cystinosin is easily accessible for transport experiments as it now faces the extracellular medium. Our model provides the first direct evidence that cystinosin transports cystine. Deletion of the lysosomal sorting motif localized in the third cytoplasmic loop was not used because it abolished transport activity (data not shown). It could be argued that cystinosin interacts with and regulates a distinct, as yet unknown, cystine transporter. However, the amount of cystine uptake correlated with the amount of cystinosin at the plasma membrane (Figure 8) and a point mutation inducing infantile cystinosis was sufficient to abolish cystine transport. Therefore, our results strongly support the conclusion that cystinosin is the lysosomal cystine transporter. The cystine transport mediated by recombinant cystinosin is consistent with previous observations on isolated lysosomes. Cystinosin-mediated cystine transport is saturable and follows Michaelis–Menten kinetics with a mean KM of 278 ± 49 μM. Similarly, countertransport experiments (in which externally added labelled cystine is exchanged for intralysosomal cystine) on lysosomes purified from human leukocytes (Gahl et al., 1983) and mouse L-929 fibroblasts (Greene et al., 1990) resulted in a KM of 500 and 270 μM, respectively. Both the recombinant and native transporters display a high selectivity for L-cystine (see Table I for a comparison). The fact that cystine uptake is not, or only moderately, inhibited by 10 mM L-glutamate on one hand, and 10 mM L-leucine or L-phenylalanine on the other hand, differentiates cystinosin from the two known plasma membrane cystine transporters 4F2hc/xCT (Sato et al., 1999) and rBAT/b°,+AT (Chairoungdua et al., 1999; Feliubadalo et al., 1999; Pfeiffer et al., 1999), respectively (for review see Palacin et al., 1998, 2001). Moreover, the affinity of cystinosin for cystine (KM = 278 μM) is lower than that of recombinant xCT (KM = 81 ± 13 μM) or b°,+AT (KM = 41 ± 20 μM) expressed in mammalian cell lines (Pfeiffer et al., 1999; Shih and Murphy, 2001). We observed a moderate affinity of cystinosin for L-cysteine (IC50 = 1.5 mM). However, when cysteine and cystine transport were compared at similar levels of occupancy, no cysteine uptake was detected (Figure 6C). Since th