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Simultaneous Determination of Levodopa, Carbidopa, Entacapone, Tolcapone, 3- O -Methyldopa and Dopamine in Human Plasma by an Hplc–MS/MS Method

2015; Future Science Ltd; Volume: 7; Issue: 2 Linguagem: Inglês

10.4155/bio.14.230

1757-6199

Rômulo Pereira Ribeiro, João Cleverson Gasparetto, Raquel de Oliveira Vilhena, Thais Martins Guimarães de Francisco, Cleverson Antônio Ferreira Martins, Marco André Cardoso, Roberto Pontarolo, Katherine Athayde Teixeira de Carvalho,

Electrochemical sensors and biosensors

Background: In this study, we developed and validated a HPLC–MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. Results & methodology: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD 0.99), precise (RSD < 11.3%), accurate (RE < 11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®. Conclusion: The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.