Comparative Photostability Studies of BODIPY and Fluorescein Dyes by Using Fluorescence Correlation Spectroscopy
2008; Wiley; Volume: 9; Issue: 14 Linguagem: Inglês
10.1002/cphc.200800299
ISSN1439-7641
AutoresBabette Hinkeldey, Alexander Schmitt, Gregor Jung,
Tópico(s)Spectroscopy and Quantum Chemical Studies
ResumoIn single-molecule applications, the photostability of fluorescent molecules is a key parameter. We apply fluorescence correlation spectroscopy to compare the photostability of four fluorescein and four borondipyrromethene (BODIPY) dyes of similar structure but different triplet yields. The latter class of dyes are more stable. In the kinetic analysis the, diffusion and photobleaching are treated as competitive processes. Corrections, which account for saturation and for experimental artefacts, are achieved solely by using experimental data. Photobleaching is found to occur mainly through the first excited singlet state S(1), in contrast to previous findings.
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