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Effects of an epidermal growth factor receptor‐based cancer vaccine on wound healing and inflammation processes in murine experimental models

2012; Wiley; Volume: 11; Issue: 1 Linguagem: Inglês

10.1111/j.1742-481x.2012.01074.x

1742-481X

Dasha Fuentes, Lewis Chacón, Á. Casacó, Nuris Ledón, Nidia Fernández, Arianna Iglesias, Diana R. Hernández, Belinda Sánchez Pérez, Rolando Pérez,

Toxin Mechanisms and Immunotoxins

Anti‐epidermal growth factor receptor (EGFR) therapies have been proven clinically effective for a variety of epithelial tumours. Vaccination of mice with the extracellular domain (ECD) of autologous EGFR overcomes the tolerance to self‐EGFR and has antimetastatic effect on EGFR+ tumour. Because EGF/EGFR‐signalling plays an important role in the inflammation stage of wound healing, the main objective of this study was to explore the possible role of murine (m) EGFR‐ECD vaccine in the croton‐oil‐induced ear oedema and wound healing process in mice as autologous experimental models, mimicking the possible post‐surgical wound complication in patients treated with human EGFR‐ECD/VSSP vaccine. Mice were intramuscularly immunised four times; biweekly with the mEGFR‐ECD/VSSP/Mont. Seven days later, an 8 mm diameter, full‐thickness skin wound was created on the back of each animal. Immunisation induced a strong specific humoral response against the mEGFR‐ECD protein and a DTH dose–response curve but interestingly, animals treated with mEGFR‐ECD/VSSP/Mont had similar inflammatory and healing speed responses compared to control ones. These data suggest that application of mEGFR‐ECD/VSSP vaccine as a therapeutic approach in cancer patients could not elicit a poor healing process after surgery.