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BIBTEX RIS

Comparison of a Semiautomated Commercial Repetitive-Sequence-Based PCR Method with Spoligotyping, 24-Locus Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Typing, and Restriction Fragment Length Polymorphism-Based Analysis of IS 6110 for Mycobacterium tuberculosis Typing

2014; American Society for Microbiology; Volume: 52; Issue: 11 Linguagem: Inglês

10.1128/jcm.02226-14

ISSN

1098-660X

Autores

Florence Brossier, Christophe Sola, G. Millot, Vincent Jarlier, Nicolas Véziris, Wladimir Sougakoff,

Tópico(s)

Infectious Diseases and Tuberculosis

Resumo

Fifty-two multidrug-resistant isolates of Mycobacterium tuberculosis representative of the currently predominant lineages in France were analyzed using repetitive-sequence-based PCR (rep-PCR) DiversiLab (DL), spoligotyping, 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing (MIRU-VNTR), and restriction fragment length polymorphism of IS6110 (IS6110-RFLP). DL, as opposed to MIRU-VNTR and IS6110-RFLP analysis, did not allow discrimination among half of the isolates, an indication of comparatively lower resolving power.

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