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Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA

1998; Elsevier BV; Volume: 91; Issue: 3 Linguagem: Inglês

10.1016/s0379-0738(97)00196-5

1872-6283

Yukihiro Shoyama, Fumiko Kawachi, Hiroyuki Tanaka, Rie Nakai, Toshiro Shibata, Kozaburo Nishi,

Cassava research and cyanide

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.