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Validation of protein crystal structures

2000; Wiley; Volume: 56; Issue: 3 Linguagem: Inglês

10.1107/s0907444999016364

1399-0047

Gerard J. Kleywegt,

Protein purification and stability

Since the process of building and refining a model of a biomacromolecule based on crystallographic data is subjective, quality-control techniques are required to assess the validity of such models. During the 1990s, much experience was gained; the methods used and some of the lessons learned are reviewed here. In addition, an extensive compendium of quality criteria and quality-control methods that are or have been used to validate models of biomacromolecules has been compiled. The emphasis in this compendium is on the validation of protein crystal structures.