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Expression and Functional Properties of the Streptococcus intermedius Surface Protein Antigen I/II

2001; American Society for Microbiology; Volume: 69; Issue: 7 Linguagem: Inglês

10.1128/iai.69.7.4647-4653.2001

1098-5522

Fernanda C. Petersen, Sylvie Brassart‐Pasco, Joëlle Ogier, J.P. Klein, S. Assev, A. Aa. Scheie,

Antimicrobial Resistance in Staphylococcus

Streptococcus intermedius is associated with deep-seated purulent infections. In this study, we investigated expression and functional activities of antigen I/II in S. intermedius. The S. intermedius antigen I/II appeared to be cell surface associated, with a molecular mass of approximately 160 kDa. Northern blotting indicated that the S. intermedius NCTC 11324 antigen I/II gene was transcribed as a monocistronic message. Maximum expression was seen during the early exponential phase. Insertional inactivation of the antigen I/II gene resulted in reduced hydrophobicity during early exponential phase, whereas no effect was detected during mid- and late exponential phases. Binding to human fibronectin and laminin was reduced in the isogenic mutant, whereas binding to human collagen types I and IV and to rat collagen type I was not significant for either the wild type or the mutant. Compared to the wild type, the capacity of the isogenic mutant to induce interleukin 8 (IL-8) release by THP-1 monocytic cells was significantly reduced. The results indicate that the S. intermedius antigen I/II is involved in adhesion to human receptors and in IL-8 induction.