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An Automated Sample Preparation System for Large-Scale DNA Sequencing

1999; Cold Spring Harbor Laboratory Press; Volume: 9; Issue: 5 Linguagem: Inglês

10.1101/gr.9.5.457

1549-5469

Andre Marziali, T. D. Willis, Nancy A. Federspiel, Ronald W. Davis,

Gene expression and cancer classification

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.