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In vitro andIn vivo anthelmintic activity ofNicotiana tabacum L. leaves against gastrointestinal nematodes of sheep

2006; Wiley; Volume: 20; Issue: 1 Linguagem: Inglês

10.1002/ptr.1800

1099-1573

Zafar Iqbal, Muhammad Lateef, Abdul Jabbar, Muhammad Nabeel Ghayur, Anwarul Hassan Gilani,

Animal Nutrition and Physiology

Phytotherapy ResearchVolume 20, Issue 1 p. 46-48 Research Article In vitro and In vivo anthelmintic activity of Nicotiana tabacum L. leaves against gastrointestinal nematodes of sheep Zafar Iqbal, Corresponding Author Zafar Iqbal zafaruaf@yahoo.com Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanDepartment of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, Pakistan.Search for more papers by this authorMuhammad Lateef, Muhammad Lateef Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanSearch for more papers by this authorAbdul Jabbar, Abdul Jabbar Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanSearch for more papers by this authorMuhammad Nabeel Ghayur, Muhammad Nabeel Ghayur Department of Biological and Biochemical Sciences, The Aga Khan University Medical College, Karachi 74800, PakistanSearch for more papers by this authorAnwarul Hassan Gilani, Anwarul Hassan Gilani Department of Biological and Biochemical Sciences, The Aga Khan University Medical College, Karachi 74800, PakistanSearch for more papers by this author Zafar Iqbal, Corresponding Author Zafar Iqbal zafaruaf@yahoo.com Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanDepartment of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, Pakistan.Search for more papers by this authorMuhammad Lateef, Muhammad Lateef Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanSearch for more papers by this authorAbdul Jabbar, Abdul Jabbar Department of Veterinary Parasitology, University of Agriculture, Faisalabad 38040, PakistanSearch for more papers by this authorMuhammad Nabeel Ghayur, Muhammad Nabeel Ghayur Department of Biological and Biochemical Sciences, The Aga Khan University Medical College, Karachi 74800, PakistanSearch for more papers by this authorAnwarul Hassan Gilani, Anwarul Hassan Gilani Department of Biological and Biochemical Sciences, The Aga Khan University Medical College, Karachi 74800, PakistanSearch for more papers by this author First published: 05 January 2006 https://doi.org/10.1002/ptr.1800Citations: 38AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The in vitro and in vivo anthelmintic activity of Nicotiana tabacum L. leaves was studied to rationalize its traditional use. Live Haemonchus contortus were used to assess the in vitro anthelmintic effect of a crude aqueous extract (CAE) and a methanol extract (CME) of N. tabacum. The in vitro inhibitory effect of both the extracts was evident from the paralysis and/or mortality of worms noted at 6 h post-exposure. For the in vivo studies, CAE and CME were administered in increasing doses (1.0–3.0 g/kg) to sheep naturally infected with mixed species of gastrointestinal nematodes. A maximum reduction of 73.6% in eggs per gram (EPG) of faeces was recorded on day 5 post-treatment with CME (3.0 g/kg) while the same dose of CAE showed a 49.4% reduction. Levamisole (7.5 mg/kg), a standard anthelmintic agent, showed a 99.6% reduction in EPG. These data show that the aqueous and methanol extracts of Nicotiana tabacum exhibit dose-dependent anthelmintic activity both in vitro and in vivo, thus justifying its use in the traditional medicine system of Pakistan. Copyright © 2006 John Wiley & Sons, Ltd. Citing Literature Volume20, Issue1January 2006Pages 46-48 RelatedInformation