Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line
2004; Asian-Australasian Association of Animal Production Societies; Volume: 17; Issue: 10 Linguagem: Inglês
10.5713/ajas.2004.1338
ISSN1976-5517
AutoresXiongtu Zhou, Yunn‐Hwa Ma, Weijun Guan, Dan Zhao,
Tópico(s)Molecular Biology Techniques and Applications
ResumoThe Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique.The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%.Confirmed by isoenzyme analysis, this cell line had no cross-contamination.Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative.This newly established cell line meets all the standard quality controls of ATCC.It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.(
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