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Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool

2013; Oxford University Press; Volume: 26; Issue: 10 Linguagem: Inglês

10.1093/protein/gzt047

1741-0134

Yingda Xu, William P. Roach, T. Sun, Tushar Jain, Bianka Prinz, Tian Yu, John Torrey, J.B. Thomas, Piotr Bobrowicz, Maximiliano Vásquez, K. Dane Wittrup, Eric Krauland,

Viral Infectious Diseases and Gene Expression in Insects

Low expression, poor solubility, and polyspecificity are significant obstacles that have impeded the development of antibodies discovered from in vitro display libraries. Current biophysical characterization tools that identify these ‘developability’ problems are typically only applied after the discovery process, and thus limited to perhaps a few hundred candidates. We report a flow cytometric assay using a polyspecificity reagent (PSR) that allows for the identification and counter selection of polyspecific antibodies both during and after the selection process. The reported assay correlates well with cross-interaction chromatography, a surrogate for antibody solubility, as well as a baculovirus particle enzyme-linked immunosorbent assay, a surrogate for in vivo clearance. However, unlike these assays, PSR labeling is compatible both with screening of individual antibodies as well as selections of large antibody libraries. To this end, we demonstrate the ability to counter-select against polyspecificity while enriching for antigen affinity from a diverse antibody library, which enables simultaneous evolution of both antigen binding and superior non-target-related properties during the discovery process.