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Murine Bladder Imaging by 2-Photon Microscopy: An Experimental Study of Morphology

2014; Lippincott Williams & Wilkins; Volume: 192; Issue: 3 Linguagem: Inglês

10.1016/j.juro.2014.03.103

1527-3792

Anna Schueth, Marc A. M. J. van Zandvoort, Wim A. Buurman, Gommert A. van Koeveringe,

Heat shock proteins research

No AccessJournal of UrologyInvestigative Urology1 Sep 2014Murine Bladder Imaging by 2-Photon Microscopy: An Experimental Study of Morphology Anna Schueth, Marc A.M.J. van Zandvoort, Wim A. Buurman, and Gommert A. van Koeveringe Anna SchuethAnna Schueth Department of Urology, Maastricht University Medical Center, Maastricht University, Maastricht, The Netherlands School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands More articles by this author , Marc A.M.J. van ZandvoortMarc A.M.J. van Zandvoort Department of Genetics and Cell Biology-Molecular Cell Biology, School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands Institute for Molecular Cardiovascular Research, Rheinisch-Westfälische Technische Hochschule Aachen University of Aachen, Aachen, Germany More articles by this author , Wim A. BuurmanWim A. Buurman School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands More articles by this author , and Gommert A. van KoeveringeGommert A. van Koeveringe Department of Urology, Maastricht University Medical Center, Maastricht University, Maastricht, The Netherlands School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands Financial interest and/or other relationship with Astellas. More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.03.103AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We developed 2-photon laser scanning microscopy analysis of the native murine bladder. Materials and Methods: Bladder tissue from wild-type mice was imaged by 2-photon laser scanning microscopy autofluorescence and second harmonic generation microscopy. Bladder wall layers and structures were analyzed using differences in color, size, shape and morphology. Results: Autofluorescence of the urothelium, nerve structures and muscles was visible in the green spectral channel due to autofluorochromes such as NAD(P)H and elastin. Second harmonic generation of collagen was seen in the blue spectral channel. Imaging from the mucosal side revealed umbrella cells at 0 and 30 μm, of which the high cellular NAD(P)H content allows autofluorescence detection. Below that a network-like connective tissue layer was visualized up to 50 μm that contained vessels with a diameter of 10 to 40 μm and nerves with a diameter of 1 to 6 μm. Imaging from the adventitial side revealed a radiant collagen layer covered with nerves and macrophages at 0 to 20 μm. Below at 20 to 25 μm we visualized a thick muscle layer containing elastic fibers and macrophages. 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Google Scholar © 2014 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byCartwright R, Franklin L, Tikkinen K, Kalliala I, Miotla P, Rechberger T, Offiah I, McMahon S, O’Reilly B, Lince S, Kluivers K, Post W, Poelmans G, Palmer M, Wessells H, Wong A, Kuh D, Kivimaki M, Kumari M, Mangino M, Spector T, Guggenheim J, Lehne B, De Silva N, Evans D, Lawlor D, Karhunen V, Männikkö M, Marczak M, Bennett P, Khullar V, Järvelin M and Walley A (2021) Genome-Wide Association Study Identifies Two Novel Loci Associated with Female Stress and Urgency Urinary IncontinenceJournal of Urology, VOL. 206, NO. 3, (679-687), Online publication date: 1-Sep-2021. Volume 192Issue 3September 2014Page: 973-980Supplementary Materials Advertisement Copyright & Permissions© 2014 by American Urological Association Education and Research, Inc.Keywordsurinary bladdermicroscopylasersoptical imagingmiceAcknowledgmentsJeroen Hameleers provided technical support. Dr. Wilfred Germeraad provided advice and MOMA-1 for macrophage staining. Bart Biallosterski provided figure 1, C. Bart Spronck assisted with nerve tracking and provided figure 7, B.Metrics Author Information Anna Schueth Department of Urology, Maastricht University Medical Center, Maastricht University, Maastricht, The Netherlands School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands More articles by this author Marc A.M.J. van Zandvoort Department of Genetics and Cell Biology-Molecular Cell Biology, School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands Institute for Molecular Cardiovascular Research, Rheinisch-Westfälische Technische Hochschule Aachen University of Aachen, Aachen, Germany More articles by this author Wim A. Buurman School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands More articles by this author Gommert A. van Koeveringe Department of Urology, Maastricht University Medical Center, Maastricht University, Maastricht, The Netherlands School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands Financial interest and/or other relationship with Astellas. More articles by this author Expand All Advertisement PDF downloadLoading ...