Leukemic cell death induced by antithymocyte globulin
2005; Elsevier BV; Volume: 29; Issue: 7 Linguagem: Inglês
10.1016/j.leukres.2002.11.001
ISSN1873-5835
AutoresAyami Yoshimi, Masahumi Ito, Seiji Kojima,
Tópico(s)RNA Interference and Gene Delivery
ResumoWe studied the cytotoxic effects of antithymocyte globulin (ATG) in leukemic cells obtained from five patients with acute T lymphoblastic leukemia or precursor T lymphoblastic leukemia. ATG by itself killed leukemic cells in a dose-dependent manner. Simultaneous incubation with human AB or baby rabbit serum resulted in increased cytolysis of leukemic cells. These results suggested the presence of both direct and complement-mediated cytolysis. We also examined apoptotic cell death using Annexin-V. Cell incubation with ATG increased Annexin-V binding significantly compared with horse IgG (50.3+/-7.6% versus 95.7+/-1.8%, p = or < 0.0001). However, ATG did not induce apparent DNA fragmentation in a human T-ALL cell line. Neither anti-Fas MoAb (ZB4) nor a broad caspase inhibitor (z-VAD FMK) prevented this increase in Annexin-V binding. These results suggest that ATG induces leukemic cell death in a Fas/Fas-ligand- and caspase-independent manner.
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