Produção Nacional
Revisado por pares
Resolution of multiclonal infections of Trypanosoma cruzi from naturally infected triatomine bugs and from experimentally infected mice by direct plating on a sensitive solid medium
2006; Elsevier BV; Volume: 37; Issue: 1 Linguagem: Inglês
10.1016/j.ijpara.2006.08.002
ISSN1879-0135
AutoresMatthew Yeo, Michael D. Lewis, H. Carrasco, Nidia Acosta, Martin Llewellyn, Sebastião Aldo da Silva Valente, Vera de Costa Valente, Antonieta Rojas de Árias, Michael A. Miles,
Tópico(s)Synthesis and Biological Evaluation
ResumoThe isolation of biological clones of Trypanosoma cruzi by microscopically dispensing individual organisms or by serial dilution is laborious and time consuming. The inability to resolve mixed T. cruzi infections, from vectors and hosts, and to isolate clones of slow growing genotypes by efficient plating on solid media, has hindered characterisation studies and downstream applications. We have devised and validated a sensitive, solid medium plating technique for rapid in vitro isolation of clones representative of all the recognised T. cruzi lineages (TCI, TCIIa–e), including the slow growing strain CANIII (TC IIa) and Trypanosoma rangeli, with high plating efficiencies. Furthermore, the method is effective for the isolation of clones directly from silvatic triatomine bugs and from experimentally infected mice harbouring mixed infections, allowing resolution of multiclonal infections from varied sources.
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