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Oligomeric structure of a simian virus 40 T antigen in free form and bound to DNA

1981; Elsevier BV; Volume: 148; Issue: 4 Linguagem: Inglês

10.1016/0022-2836(81)90180-7

1089-8638

R Myers, Robley C. Williams, Robert Tjian,

Plant Virus Research Studies

The structure of the D2 protein, a polypeptide structurally and functionally related to the simian virus 40 large T antigen, was examined by electron microscopy. The observed size of the protein indicates that it most commonly exists as a tetrameric assembly of its 107,000 Mr monomer. In addition to the tetramers, both monomers and dodecamers are seen. Binding of D2 protein to a fragment of simian virus 40 DNA was found to occur preferentially at the origin of replication. The major binding species of the protein is the tetramer.