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Nucleotide sequence of IS 1542 , an insertion sequence identified within VanA glycopeptide resistance elements of enterococci

1999; Oxford University Press; Volume: 173; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1999.tb13523.x

1574-6968

Ana Lúcia da Costa Darini, Marie-France I. Palepou, Neil Woodford,

Biochemical and Structural Characterization

IS1542 is an insertion sequence-like element which was originally identified in the orf2-vanR intergenic region of VanA resistance elements of glycopeptide-resistant Enterococcus faecium from hospital patients and from non-human sources in the UK. The nucleotide sequence of IS1542 was determined. It showed 81% homology with IS256 and contained an open reading frame sufficient to encode a 390 amino acid peptide predicted to have 87.2% identity with the transposase of IS256. By PCR, IS1542 was detected in the genomes of 26 of 28 (93%) VanA enterococci isolated from poultry in the UK and Ireland, but in only two of 65 (3%) glycopeptide-sensitive or VanC enterococci from hospital patients in the UK and Brazil. IS1542 may be a useful marker for evolutionary and epidemiological studies of VanA glycopeptide resistance in enterococci.