Artigo Acesso aberto Revisado por pares

Identification of 3 Phylogenetically Related HIV-1 BG Intersubtype Circulating Recombinant Forms in Cuba

2007; Lippincott Williams & Wilkins; Volume: 45; Issue: 2 Linguagem: Inglês

10.1097/qai.0b013e318046ea47

ISSN

1944-7884

Autores

María Sierra, Michael M. Thomson, David Posada, Lissette Pérez, Carlos Aragonés, Zoila González, Jorge Pérez, Gema Casado, Rafael Nájera,

Tópico(s)

Virology and Viral Diseases

Resumo

BG intersubtype recombinants represented 11.6% of HIV-1 isolates in a recent survey in Cuba based on pol sequences, most of them forming a single clade further subdivided into 3 subclades. Here, we analyze 8 near full-length genomes and 1 gag-pol sequence from epidemiologically unlinked Cuban BG recombinants from these 3 subclades (3 from each). Near full-length sequences were also obtained from 3 subtype G and 2 subtype B Cuban viruses. Phylogenetic relationships were estimated via maximum likelihood, and mosaic structures of the recombinants were inferred with the bootscanning, MaxChi, Genconv, and GARD methods. For the near full-length genomes, all recombinants formed a strongly supported clade further subdivided into the same subclades previously defined in pol. Mosaic structures were identical within each subclade and different among subclades, although 5 breakpoints were coincident among all recombinants. Individual phylogenetic trees for nonrecombinant fragments (concatenated B and G subtype segments) indicated a common ancestry for the parental viruses and their relationships to local subtype B and G strains. These results allow us to identify 3 new BG intersubtype circulating recombinant forms in Cuba derived from a common recombinant ancestor, which originated from B and G subtype parental strains circulating in Cuba.

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