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Betulin Is a Potent Anti-Tumor Agent that Is Enhanced by Cholesterol

2009; Public Library of Science; Volume: 4; Issue: 4 Linguagem: Inglês

10.1371/journal.pone.0005361

1932-6203

Franziska B. Mullauer, Jan H. Kessler, Jan Paul Medema,

ATP Synthase and ATPases Research

Betulinic Acid (BetA) and its derivatives have been extensively studied in the past for their anti-tumor effects, but relatively little is known about its precursor Betulin (BE). We found that BE induces apoptosis utilizing a similar mechanism as BetA and is prevented by cyclosporin A (CsA). BE induces cell death more rapidly as compared to BetA, but to achieve similar amounts of cell death a considerably higher concentration of BE is needed. Interestingly, we observed that cholesterol sensitized cells to BE-induced apoptosis, while there was no effect of cholesterol when combined with BetA. Despite the significantly enhanced cytotoxicity, the mode of cell death was not changed as CsA completely abrogated cell death. These results indicate that BE has potent anti-tumor activity especially in combination with cholesterol.