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Immunocytochemical localization of the dopamine transporter in human brain

1999; Wiley; Volume: 409; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9861(19990621)409

1096-9861

Brian J. Ciliax, Genny W. Drash, Julie K. Staley, Sharon Haber, Catherine J. Mobley, Gary W. Miller, Elliott J. Mufson, Deborah C. Mash, Allan I. Levey,

Receptor Mechanisms and Signaling

Journal of Comparative NeurologyVolume 409, Issue 1 p. 38-56 Immunocytochemical localization of the dopamine transporter in human brain Brian J. Ciliax, Corresponding Author Brian J. Ciliax bciliax@emory.edu Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Department of Neurology, Emory University School of Medicine, Wesley Woods Health Center, Laboratory for Brain Science, 1841 Clifton Road, NE, Atlanta, GA 30329.Search for more papers by this authorGenny W. Drash, Genny W. Drash Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorJulie K. Staley, Julie K. Staley Department of Neurology, University of Miami Brain Endowment Bank, University of Miami, Miami, Florida 33136Search for more papers by this authorSharon Haber, Sharon Haber Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorCatherine J. Mobley, Catherine J. Mobley Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorGary W. Miller, Gary W. Miller Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorElliott J. Mufson, Elliott J. Mufson Rush-Presbyterian Hospital, St. Luke's Medical Center, Chicago, Illinois 60612Search for more papers by this authorDeborah C. Mash, Deborah C. Mash Department of Neurology, University of Miami Brain Endowment Bank, University of Miami, Miami, Florida 33136Search for more papers by this authorAllan I. Levey, Allan I. Levey Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this author Brian J. Ciliax, Corresponding Author Brian J. Ciliax bciliax@emory.edu Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Department of Neurology, Emory University School of Medicine, Wesley Woods Health Center, Laboratory for Brain Science, 1841 Clifton Road, NE, Atlanta, GA 30329.Search for more papers by this authorGenny W. Drash, Genny W. Drash Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorJulie K. Staley, Julie K. Staley Department of Neurology, University of Miami Brain Endowment Bank, University of Miami, Miami, Florida 33136Search for more papers by this authorSharon Haber, Sharon Haber Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorCatherine J. Mobley, Catherine J. Mobley Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorGary W. Miller, Gary W. Miller Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this authorElliott J. Mufson, Elliott J. Mufson Rush-Presbyterian Hospital, St. Luke's Medical Center, Chicago, Illinois 60612Search for more papers by this authorDeborah C. Mash, Deborah C. Mash Department of Neurology, University of Miami Brain Endowment Bank, University of Miami, Miami, Florida 33136Search for more papers by this authorAllan I. Levey, Allan I. Levey Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322Search for more papers by this author First published: 20 May 1999 https://doi.org/10.1002/(SICI)1096-9861(19990621)409:1 3.0.CO;2-1Citations: 233AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The dopamine transporter (DAT) was localized in normal human brain tissue by light microscopic immunocytochemistry by using highly specific monoclonal antibodies. Regional distribution of DAT was found in areas with established dopaminergic circuitry, e.g., mesostriatal, mesolimbic, and mesocortical pathways. Mesencephalic DAT-immunoreactivity was enriched in the dendrites and cell bodies of neurons in the substantia nigra pars compacta and ventral tegmental area. Staining in the striatum and nucleus accumbens was dense and heterogeneous. Mesocortical DAT immunoreactivity in motor, premotor, anterior cingulate, prefrontal, entorhinal/perirhinal, insular, and visual cortices was detected in scattered varicose and a few nonvaricose fibers. Varicose fibers were relatively enriched in the basolateral and central subnuclei of amygdala, with sparser fibers in lateral and basomedial subnuclei. Double-labeling studies combining DAT and tyrosine hydroxylase (TH) immunostaining in the ventral mesencephalon showed two subpopulations of dopaminergic neurons differentiated by the presence or absence of DAT-immunoreactivity in the A9 and A10 cell groups. In other dopaminergic cell groups (A11, A13–A15), TH-positive hypothalamic neurons showed no detectable DAT-immunoreactivity. However, fine DAT-immunoreactive axons were scattered throughout the hypothalamus, particularly concentrated along the medial border, with more coarse axons present along the lateral border. These findings demonstrate that most mesotelencephalic dopamine neurons of human brain express high levels of DAT throughout their entire somatodendritic and axonal domains, whereas a smaller subpopulation of mesencephalic dopamine cells and all hypothalamic dopamine cell groups examined express little or no DAT. These data indicate that different subpopulations of dopaminergic neurons use different mechanisms to regulate their extracellular dopamine levels. J. Comp. Neurol. 409:38–56, 1999. © 1999 Wiley-Liss, Inc. Citing Literature Volume409, Issue121 June 1999Pages 38-56 RelatedInformation