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Semiautomated Turbidimetric Microbiological Assay for Determination of Cefazolin

1974; American Society for Microbiology; Volume: 5; Issue: 3 Linguagem: Inglês

10.1128/aac.5.3.223

1098-6596

Donald Pitkin, Paul Actor, Walter W. Holl, Alex Post, Jerry A. Weisbach,

Antimicrobial Resistance in Staphylococcus

The Autoturb System, a semiautomated system for photometric bioassay, was used to determine cefazolin content. Suitable conditions for the assay using Streptococcus faecalis ATCC 10541 as the indicator organism included a medium pH of 6.0 to 7.0 and an incubation time of 3 to 3.5 h at 36 C. Multiple independent assays of samples from a common batch showed the test to be highly reproducible. Accuracy of the turbidimetric assay was evaluated by comparing data obtained from a chemical (hydroxylamine) and a biological (disk diffusion) assay. The available data show the turbidimetric assay to be a rapid, accurate, and reproducible method for determining the biological activity of cefazolin samples.