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The completion of the Mammalian Gene Collection (MGC)

2009; Cold Spring Harbor Laboratory Press; Volume: 19; Issue: 12 Linguagem: Inglês

10.1101/gr.095976.109

1549-5469

Gary Temple, Daniela S. Gerhard, Rebekah S. Rasooly, Elise A. Feingold, Peter J. Good, C. Scott. Robinson, Allison Mandich, Jeffrey G. Derge, Jeanne Lewis, Debonny Shoaf, Francis S. Collins, Wonhee Jang, Lukas Wagner, Carolyn M. Shenmen, Leonie Misquitta, Carl F. Schaefer, Kenneth H. Buetow, Tom I. Bonner, Linda Yankie, Ming Ward, Lon Phan, Alex Astashyn, Garth Brown, Catherine M. Farrell, Jennifer Hart, Melissa Landrum, B. Maidak, Michael R. Murphy, Terence D. Murphy, Bhanu Rajput, Lillian D. Riddick, David Webb, Janet A. Weber, Wendy Wu, Kim D. Pruitt, Donna Maglott, Adam Siepel, Broňa Brejová, Mark Diekhans, Rachel Harte, Robert Baertsch, Jim Kent, David Haussler, Michael R. Brent, Laura Langton, Charles L.G. Comstock, Michael Stevens, Chaochun Wei, Marijke J. van Baren, Kourosh Salehi‐Ashtiani, Ryan R. Murray, Lila Ghamsari, Elizabeth Mello, Chenwei Lin, Christa Pennacchio, Kirsten Schreiber, Nicole Shapiro, Amber Marsh, Elizabeth Pardes, Troy Moore, Anita Lebeau, Mike Muratet, Blake A. Simmons, David Kloske, Stephanie Sieja, James R. Hudson, Praveen Sethupathy, Michael Brownstein, Narayan Bhat, Joseph Lazar, Howard J. Jacob, C. E. Gruber, Mark R. Smith, John D. McPherson, Angela Garcia, Preethi H. Gunaratne, Jiaqian Wu, Donna M. Muzny, Richard A. Gibbs, Alice Young, Gerard G. Bouffard, Robert W. Blakesley, Jim Mullikin, Eric D. Green, Mark Dickson, Álex Rodríguez, Jane Grimwood, Jeremy Schmutz, R Myers, Martin Hirst, Thomas Zeng, Kane Tse, Michelle Moksa, Merinda Deng, Kevin Ma, Diana Mah, Johnson Pang, Greg Taylor, Eric Chuah, Athena Deng, Keith Fichter, Anne Go, Stephanie J. Lee, Jing Wang, Malachi Griffith, Ryan D. Morin, Richard A. Moore, Michael Mayo, Sarah Munro, Susan Wagner, Steven J.M. Jones, Robert A. Holt, Marco A. Marra, Lu Sun, Shuwei Yang, James Hartigan, Marcus Graf, Ralf Wagner, Stanley Letovksy, Jacqueline C. Pulido, Keith Robison, Dominic Esposito, James L. Hartley, Vanessa Wall, Ralph F. Hopkins, Osamu Ohara, Stefan Wiemann,

Genomics and Phylogenetic Studies

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.