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Probing the Role of the DXDD Motif in Class II Diterpene Cyclases

2007; Wiley; Volume: 8; Issue: 8 Linguagem: Inglês

10.1002/cbic.200700045

1439-7633

Sladjana Prišić, Jianjun Xu, Robert M. Coates, Reuben J. Peters,

Sesquiterpenes and Asteraceae Studies

On common ground. An epoxy analogue of geranylgeranyl diphosphate was used together with site-directed mutagenesis to provide evidence that the DXDD motif in class II diterpene cyclases is involved in CC double-bond protonation (see scheme). This function is similar to the role of the DXDD motif found in the otherwise unrelated triterpene cyclases, which also catalyze CC protonation initiated cyclization.