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Observation of peptide differences between cancer and control in gastric juice

2008; Wiley; Volume: 2; Issue: 1 Linguagem: Inglês

10.1002/prca.200780066

1862-8354

Wei-Chao Chang, Ping-I Hsu, Yuan-Yan Chen, Michael Hsiao, Pei‐Jung Lu, Chung‐Hsuan Chen,

Protein Hydrolysis and Bioactive Peptides

Abstract Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI‐TOF‐MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were clear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α‐1‐antitrypsin fragment, have been identified by mass spectrometric analysis and immuno‐deplete assay with antibodies.