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Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae)

2011; Research Foundation of Ribeirão Preto; Volume: 10; Issue: 4 Linguagem: Inglês

10.4238/2011.november.1.1

ISSN

1676-5680

Autores

Raquel Rinke, Andrea Soares‐Costa, Fernando P.P. Fonseca, Lidiane Cordeiro de Almeida, Ítalo Delalibera, Flávio Henrique‐Silva,

Tópico(s)

Insect behavior and control techniques

Resumo

The sugarcane weevil, Sphenophorus levis, is a widespread sugarcane pest in Brazil.Sphenophorus levis may depend on microorganisms that inhabit its intestinal tract.We examined the diversity of the gut microbiota of S. levis, which was characterized using culturedependent and culture-independent methods.Analysis of 16S rRNA amplified directly from the gut community revealed the presence of 14 genera, one group from the Candidatus category, one uncultured group assigned to the family Flavobacteriaceae, and one uncultured group assigned to the family Enterobacteriaceae; all of them are members of the Alpha-Proteobacteria, Beta-Proteobacteria, Gamma-Proteobacteria, Firmicutes, and Bacteroidetes phyla.Microorganisms isolated through culture-dependent methods were classified according to morphological ©FUNPEC-RP www.funpecrp.com.

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