High performance liquid chromatography and nuclear magnetic resonance analysis of longipinene derivatives from Stevia subpubescens var. intermedia
1991; Wiley; Volume: 2; Issue: 2 Linguagem: Inglês
10.1002/pca.2800020207
ISSN1099-1565
AutoresPedro Joseph‐Nathan, Carlos M. Cerda‐García‐Rojas, Salmantina Castrejón, Luisa U. Román, Juan D. Hernández,
Tópico(s)Olfactory and Sensory Function Studies
ResumoAbstract The methodology for the separation of complex mixtures of longipin‐2‐ene‐7β, 9α‐diol‐1‐one diesters using high performance liquid chromatography followed by the identification of individual components by pulse Fourier transform 1 H nuclear magnetic resonance spectroscopy is described. The identification methodology takes advantage of the spectral comparison between the isolated natural product and a wide series of previously prepared longipinene derivatives, and is applied to the study of the extracts of Stevia subpubescens var. intermedia which contain 11 previously isolated longipinene diesters and the new natural product longipin‐2‐ene‐7β, 9α‐diol‐1‐one 7‐seneciate‐9‐angelate.
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