Highly efficient protein trans ‐splicing by a naturally split DnaE intein from Nostoc punctiforme
2006; Wiley; Volume: 580; Issue: 7 Linguagem: Inglês
10.1016/j.febslet.2006.02.045
ISSN1873-3468
AutoresHideo Iwaï, Sara Züger, Jennifer Jin, Pui‐Hang Tam,
Tópico(s)RNA modifications and cancer
ResumoProtein trans ‐splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme ( Npu DnaE) was demonstrated here with non‐native exteins in Escherichia coli . Npu DnaE possesses robust trans ‐splicing activity with an efficiency of >98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 ( Ssp DnaE). Both the N‐ and C‐terminal parts of the split Npu DnaE intein can be substituted with the corresponding fragment of Ssp DnaE without loss of trans ‐splicing activity. Protein splicing with the Npu DnaE N is also more tolerant of amino acid substitutions in the C‐terminal extein sequence.
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