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A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication

2003; Cold Spring Harbor Laboratory Press; Volume: 17; Issue: 9 Linguagem: Inglês

10.1101/gad.1070003

1549-5477

Yumiko Kubota, Youhei Takase, Yasunori Komori, Yoshitami Hashimoto, Toshiaki Arata, Yoichiro Kamimura, Hiroyuki Araki, Haruhiko Takisawa,

Bacterial Genetics and Biotechnology

We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.