Portal de Periódicos da CAPES

Effect of Glutaraldehyde on the Outer Layers of Escherichia coli

1972; Wiley; Volume: 35; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2672.1972.tb03690.x

2056-5232

T. J. Munton, A.D. Russell,

Pharmaceutical and Antibiotic Environmental Impacts

S ummary : Sodium lauryl sulphate (SLS) at pH 3 and 8 lysed cell walls of Escherichia coli. Pretreatment with glutaraldehyde at pH 3 and at pH 8 prevented this lysis. SLS induced maximum lysis of E. coli cells at 40°; pretreatment of cells with glutaraldehyde prevented this lysis also. Electrophoretic studies indicated that glutaraldehyde accumu lated on the surface of E. coli cells more rapidly in acid than in alkaline conditions, and that it blocked amino groups on the surface layer of Bacillus subtilis spores. The relationship of these findings to the bactericidal efficiency of glutaraldehyde in acid and alkaline solution is discussed.