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Towards Stratifying Ischemic Components by Cardiac MRI and Multifunctional Stainings in a Rabbit Model of Myocardial Infarction

2014; Ivyspring International Publisher; Volume: 4; Issue: 1 Linguagem: Inglês

10.7150/thno.7188

1838-7640

Yuanbo Feng, Feng Chen, Zhanlong Ma, Frederik DeKeyzer, Jie Yu, Yi Xie, Marlein Miranda Cona, Raymond Oyen, Yicheng Ni,

Cardiovascular Function and Risk Factors

Objectives: We sought to identify critical components of myocardial infarction (MI) including area at risk (AAR), MI-core and salvageable zone (SZ) by using cardiac magnetic resonance imaging (cMRI) and multifunctional stainings in rabbits.Materials and Methods: Fifteen rabbits received 90-min coronary artery (CA) ligation and reopening to induce reperfused MI.First-pass perfusion weighted imaging (PWI 90' ) was performed immediately before CA reperfusion.Necrosis avid dye Evans blue (EB) was intravenously injected for later MI-core detection.One-day later, cMRI with T2-weighted imaging (T2WI), PWI 24h and delayed enhancement (DE) T1WI was performed at a 3.0T clinical scanner.The heart was excised and CA was re-ligated with aorta infused by red-iodized-oil (RIO).The heart was sliced into 3-mm sections for digital radiography (DR), histology and planimetry with myocardial salvage index (MSI) and perfusion density rate (PDR) calculated.Results: There was no significant difference between MI-cores defined by DE-T1WI and EB-staining (31.13±8.55%vs 29.80±7.97%;p=0.74).The AAR was defined similarly by PWI 90' (39.93±9.51%),RIO (38.82±14.41%)and DR (38.17±15.98%),underestimated by PWI 24h (36.44±5.31%),but overestimated (p<0.01) by T2WI (56.93±8.87%).Corresponding MSI turned out to be 24.17±9.5% (PWI 90' ), 21.97±9.41%(DR) and 22.68±9.65%(RIO), which were significantly (p<0.01)higher and lower than that with PWI 24h (15.15±7.34%)and T2WI (45.52±7.5%)respectively.The PDR differed significantly (p<0.001) between normal myocardium (350.6±33.1%)and the AAR (31.2±15%), suggesting 11-times greater blood perfusion in normal myocardium over the AAR.Conclusion: The introduced rabbit platform and new staining techniques together with the use of a 3.0T clinical scanner for cMRI enabled visualization of MI components and may contribute to translational cardiac imaging research for improved theranostic management of ischemic heart disease.