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Mass Spectrometric Imaging of Ginsenosides Localization in Panax ginseng Root

2010; World Scientific; Volume: 38; Issue: 03 Linguagem: Inglês

10.1142/s0192415x10008007

1793-6853

Shu Taira, Ryuzo Ikeda, Naohiko Yokota, Issey Osaka, Manabu Sakamoto, Mitsuro Kato, Yuko Sahashi,

Natural product bioactivities and synthesis

We performed mass spectrometric imaging (MSI) to localize ginsenosides (Rb(1), Rb(2) or Rc, and Rf) in cross-sections of the Panax ginseng root at a resolution of 100 microm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Tandem mass spectrometry (MS/MS) of alkali metal-adducted ginsenoside ions revealed structural information of the corresponding saccharides and aglycone. MALDI-MSI confirmed that ginsenosides were located more in the cortex and the periderm than that in the medulla of a lateral root. In addition, it revealed that localization of ginsenosides in a root tip (diameter, 2.7 mm) is higher than that in the center of the root (diameter, 7.3 mm). A quantitative difference was detected between localizations of protopanaxadiol-type ginsenoside (Rb(1), Rb(2), or Rc) and protopanaxatriol-type ginsenoside (Rf) in the root. This imaging approach is a promising technique for rapid evaluation and identification of medicinal saponins in plant tissues.