Isolation of the Oleaginous Yeasts from the Soil and Studies of Their Lipid-Producing Capacities
2009; Linguagem: Inglês
Autores
Li-Xia Pan, Dengfeng Yang, Li Shao, Wei Li, Guiguang Chen, Zhiqun Liang,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoSummary D-xylose is one of the most abundant pentose sugars in nature. To isolate oleaginous yeasts that can utilize xylose from diverse soil samples, soils from a litchi orchard, longan orchard, carambola orchard, and woods were collected, yeasts were isolated by the glycerol enrichment and their xylose-assimilating capacities were measured. A subset of these isolates was grown in nitrogen-limited media and then screened for potential oleaginous yeasts by the Sudan Black B staining, after which their lipid-producing capacities were studied. There were 13 strains of oleaginous yeasts identified, and a rapid microbiological assay was provided to exploit microbial lipids that may one day be used as biodiesels or cocoa butter substitute.
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