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Application of Dried Blood Spot Specimens for Serologic Subtyping of Human Immunodeficiency Virus Type 1 in Thailand

1999; American Society for Microbiology; Volume: 37; Issue: 3 Linguagem: Inglês

10.1128/jcm.37.3.804-806.1999

1098-660X

Penprapa Chanbancherd, Arthur E. Brown, Rapee Trichavaroj, P Tienamporn, P. Puthakird, Nukool Limpairojn, Thomas C. VanCott, Mark de Souza,

HIV-related health complications and treatments

ABSTRACT Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.