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Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome

2014; Springer Nature; Volume: 6; Issue: 8 Linguagem: Inglês

10.15252/emmm.201404044

1757-4684

Vladimir Camarena, Lei Cao, Clemer Abad, Alexander J. Abrams, Yaima Toledo, Kimi Araki, Masatake Araki, Katherina Walz, Juan I. Young,

Congenital heart defects research

Abstract 2q23.1 microdeletion syndrome is characterized by intellectual disability, motor delay, autistic‐like behaviors, and a distinctive craniofacial phenotype. All patients carry a partial or total deletion of methyl‐CpG‐binding domain protein 5 ( MBD 5 ), suggesting that haploinsufficiency of this gene is responsible for the phenotype. To confirm this hypothesis and to examine the role of MBD 5 in vivo, we have generated and characterized an Mbd5 gene‐trap mouse model. Our study indicates that the Mbd5 +/ GT mouse model recapitulates most of the hallmark phenotypes observed in 2q23.1 deletion carriers including abnormal social behavior, cognitive impairment, and motor and craniofacial abnormalities. In addition, neuronal cultures uncovered a deficiency in neurite outgrowth. These findings support a causal role of MBD 5 in 2q23.1 microdeletion syndrome and suggest a role for MBD 5 in neuronal processes. The Mbd5 +/ GT mouse model will advance our understanding of the abnormal brain development underlying the emergence of 2q23.1 deletion‐associated behavioral and cognitive symptoms.