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Cytokine Signatures of Innate and Adaptive Immunity in 17DD Yellow Fever Vaccinated Children and Its Association With the Level of Neutralizing Antibody

2011; Oxford University Press; Volume: 204; Issue: 6 Linguagem: Inglês

10.1093/infdis/jir439

1537-6613

Maria Luiza-Silva, Ana Carolina Campi‐Azevedo, Maurício Azevedo Batista, Marco A. Martins, Renato Sathler Avelar, Denise Silveira-Lemos, Luiz Antônio Bastos Camacho, Reinaldo de Menezes Martins, Maria de Lourdes de Sousa Maia, Roberto Farias, Marcos da Silva Freire, Ricardo Galler, Akira Homma, José Geraldo Leite Ribeiro, Jandira Aparecida Campos Lemos, Maria Auxiliadora‐Martins, Silvana Maria Elói-Santos, Andréa Teixeira−Carvalho, Olindo Assis Martins‐Filho,

Cytomegalovirus and herpesvirus research

Background. The live attenuated yellow fever (YF) vaccines have been available for decades and are considered highly effective and one of the safest vaccines worldwide. Methods. The impact of YF-17DD-antigens recall on cytokine profiles of YF-17DD-vaccinated children were characterized using short-term cultures of whole blood samples and single-cell flow cytometry. This study enrolled seroconverters and nonseroconverters after primovaccination (PV-PRNT+ and PV-PRNT−), seroconverters after revaccination (RV-PRNT+), and unvaccinated volunteers (UV-PRNT−). Results. The analysis demonstrated in the PV-PRNT+ group a balanced involvement of pro-inflammatory/regulatory adaptive immunity with a prominent participation of innate immunity pro-inflammatory events (IL-12+ and TNF-α+ NEU and MON). Using the PV-PRNT+ cytokine signature as a reference profile, PV-PRNT− presented a striking lack of innate immunity proinflammatory response along with an increased adaptive regulatory profile (IL-4+CD4+ T cells and IL-10+ and IL-5+CD8+ T cells). Conversely, the RV-PRNT+ shifted the overall cytokine signatures toward an innate immunity pro-inflammatory profile and restored the adaptive regulatory response. Conclusions. The data demonstrated that the overall cytokine signature was associated with the levels of PRNT antibodies with a balanced innate/adaptive immunity with proinflammatory/regulatory profile as the hallmark of PV-PRNTMEDIUM+, whereas a polarized regulatory response was observed in PV-PRNT− and a prominent proinflammatory signature was the characteristic of PV-PRNTHIGH+.