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Chemical Nature of Agrocin 84 and Its Effect on a Virulent Strain of Agrobacterium tumefaciens

1976; American Society for Microbiology; Volume: 10; Issue: 3 Linguagem: Inglês

10.1128/aac.10.3.498

1098-6596

Barbara A. McCardell, Christine F. Pootjes,

Plant Genetic and Mutation Studies

Agrocin 84, produced by Agrobacterium radiobacter K84, inhibited ribonucleic acid, deoxyribonucleic acid, and protein synthesis and amino acid transport in a susceptible, virulent strain of A. tumefaciens H-38-9. Cell motility was immediately stopped by action of the agrocin, 50% of the cells were killed within 15 min of contact, and the remainder were inhibited. Agrocin 84 is trypsin and pepsin resistant, but chemical analysis indicated a small peptide with a molecular weight of 2,500 containing six different amino acids, including nine molecules of glutamine or glutamic acid and seven molecules of serine.