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Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants

2006; Elsevier BV; Volume: 9; Issue: 1 Linguagem: Inglês

10.4067/s0717-34582006000100008

0717-3458

Soledad De Nicolás-Santiago, Carlos Regalado, Blanca E. García‐Almendárez, Francisco J. Fernández, Alejandro Téllez‐Jurado, Sergio Huerta‐Ochoa,

Microbial Metabolic Engineering and Bioproduction

Mutant strains from Aspergillus niger UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship between diameter of hydrolysis zone and colony diameter. The mutant GS1-S067 showed a colony radial extension rate and a biomass growth rate [g biomass/(cm 2 h)], 1.17 times higher than that achieved by strain UAM-GS1. The high invasive capacity makes this mutant strain a promising alternative for its use in solid substrate fermentation (SSF). The morphological properties of the two mutant strains were evaluated by using scanning electron microscopy. The diameter of the sporangium of the mutant strains GS1-S059 and GS1-S067 was significantly larger ( P P