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Structure of the CAP-DNA Complex at 2.5 Å Resolution: A Complete Picture of the Protein-DNA Interface

1996; Elsevier BV; Volume: 260; Issue: 3 Linguagem: Inglês

10.1006/jmbi.1996.0409

1089-8638

Gary N. Parkinson, Christopher K. Wilson, Angelo Gunasekera, Yon W. Ebright, Richard H. Ebright, Helen M. Berman,

RNA and protein synthesis mechanisms

The crystallographic structure of the CAP-DNA complex at 3.0 Å resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 Å resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.